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Rat Macrophages (Aged), Bone Marrow (MTS-0922-JF86)

Datasheet

Overview

Description

This product is isolated from the bone marrow of pathogen-free laboratory rat. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Rat

Product Type

Rat Macrophages

Specification

Cell Source

Bone marrow

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

1 vial

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

How do these aged macrophages differ functionally from macrophages derived from young rats?

Compared with young‑donor macrophages, aged rat macrophages often exhibit reduced phagocytic capacity and distinct cytokine secretion patterns. For example, they may display elevated basal levels of inflammatory mediators yet respond less vigorously to acute stimulation. These differences are thought to contribute to impaired pathogen clearance, poor vaccine responses, and chronic inflammation in older animals. Our aged macrophages are selected and characterized to reflect these typical aging‑associated phenotypes, allowing direct comparison with young macrophage populations.

Are there any special handling considerations because the donors are aged?

Yes, macrophages from aged donors can be somewhat more sensitive to stress. It is especially important to follow the thawing protocol carefully, avoid harsh centrifugation, and minimize prolonged exposure to DMSO. Allowing the cells adequate recovery time in complete medium before starting functional assays helps them regain optimal morphology and adherence.

Can I design side-by-side experiments using aged and young macrophages from your portfolio?

Absolutely. Many researchers use our aged macrophages side‑by‑side with young rat macrophages from the same supplier to dissect age‑related changes in innate immunity.

We study immunosenescence, and these aged rat macrophages provided a very practical and relevant in vitro model.
Compared with young macrophages, they showed reduced phagocytic activity and distinct cytokine signatures, consistent with the aging literature. This direct comparison helped us connect our in vivo findings to cellular mechanisms without the need to maintain our own colony of aged animals.
The handling protocol was clear and emphasized gentle thawing and recovery, which proved important.
Once we followed those recommendations, the cells attached and spread well and remained viable long enough to complete our stimulation experiments.
Running side‑by‑side experiments with aged and young macrophages from the same supplier greatly simplified our study design.
The consistency in isolation and QC methods allowed us to attribute observed differences primarily to donor age rather than technical factors. This comparative setup has already generated several figure‑ready datasets.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.