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C57BL/6 Mouse Macrophages, Peritoneal Cavity, 6 x 10^6 (MTS-0922-JF16)

Datasheet

Overview

Description

This product is isolated from the peritoneal cavity of pathogen-free laboratory C57BL/6 mouse. 6 x 10^6 cells are delivered frozen. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

C57BL/6 Mouse Macrophages

Specification

Cell Source

Peritoneal cavity

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

6 x 10^6 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What is the functional difference between resident and elicited macrophages?

Resident macrophages represent the steady-state, "housekeeping" phenotype (often more M2-like with high F4/80 expression). Elicited macrophages are in an activated, inflammatory state. You should choose this resident population if you want to study the naïve M0 state or polarize them to M1 or M2 yourself.

Why choose the C57BL/6 strain?

C57BL/6 (B6) is the most common mouse background for genetic knockout models. These macrophages are perfect for in vitro experiments that need to match your in vivo B6-background studies, particularly for Th1-driven inflammation, infection, or cancer models.

What cell count is in this vial?

This vial provides a high cell count of 6 x 10^6 (6 million) viable cells, which is excellent for multiple experiments or large-format plates (e.g., 6-well plates).

High viability and a great cell count
These are our lab's standard for all in vitro validation work that matches our B6 mouse models. The 6M cell count is a fantastic value. They are always clean and respond beautifully to LPS/IFN-g.
Accurate cell count, and clean. The B6 background was essential. Five stars.
Used these for a co-culture assay with T-cells, and they performed flawlessly. It's so much easier than harvesting them ourselves, and the lot-to-lot consistency is much better. Highly recommend.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.