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MacroCargo™ Human PBMC-derived Macrophages with pDNA encoding IL-12 (Nanoparticle System, Magnetic cationic liposomes) (MTS-1122-YF22)

Cell Properties

Product Overview
As a therapeutic tool, macrophage cell has a great capacity for delivering cargos because of their intrinsic characteristics. This product is engineered Human PBMC-derived Macrophages carried with pDNA encoding IL-12 by Nanoparticle System-Magnetic cationic liposomes. MacroCargo™ products aim to improve the macrophage function and delivery of specific cargos. We also provide custom macrophage delivery systems based on your specific requirements.
Cell Name
PBMC-derived Macrophages
Cell Type
Primary Cell
Cell Species
Human
Cell Background
Macrophages are specialised cells involved in the detection, phagocytosis and destruction of bacteria and other harmful organisms. In addition, they can also present antigens to T cells and initiate inflammation by releasing molecules (known as cytokines) that activate other cells. Furthermore, macrophages are able to cross the biological barriers, penetrate into tumors, and accumulate particularly in hypoxic and poorly vascularized portions of a tumor.

Cargo Properties

Cargo Type
Cytokine
Specific Cargo
pDNA encoding IL-12
Cargo Common Name
IL12
Cargo Alternative Names
IL12; IL-12; Interleukin 12
Cargo Full Name
Interleukin 12
Introduction
This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.
UniprotID
GeneID
Cargo Delivery System Type
Nanoparticle System
Cargo Delivery Approach
Magnetic cationic liposomes
Nanoparticle Component
DOTAP, DSPC, cholesterol, iron oxide (II, III).

Product Properties

Applications
Improve the colonic delivery of macrophages and its therapeutic efficacy against colonic inflammatory diseases.
References
Kono, Yusuke et al. "Enhanced macrophage delivery to the colon using magnetic lipoplexes with a magnetic field." Drug deliveryvol. 26,1 (2019): 935-943. doi:10.1080/10717544.2019.1662515
Mycoplasma Testing
Negative
Sterility Testing
Negative
Shipping
Dry ice
Storage
Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C) for long term.
Handling Notes
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
Restriction
Research use only

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.

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