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CD1 Mouse Macrophages, Lung, 0.5 x 10^6 (MTS-0922-JF15)

Datasheet

Overview

Description

This product is isolated from the lung of pathogen-free laboratory CD1 mouse. 0.5 x 10^6 cells are delivered frozen. This product is negative in the tests of mycoplasma, bacteria, fungi and yeast. The expression of CD11b is positive by flow cytometry. It is guaranteed to further culture in the conditions provided by Creative Biolabs. Repeated freezing and thawing of cells is not recommended.

Applications

Numerous types of experimental manipulations, including morphological, gene expression, and physiological studies.

Species

Mouse

Product Type

CD1 Mouse Macrophages

Specification

Cell Source

Lung

Marker expression

Expressed on macrophages, CD11b plays a critical role in regulating pathogen recognition, phagocytosis, and cell survival. The expression of CD11b is positive by flow cytometry.

Status

Frozen

Formulation

Cryopreservation medium

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Medium

Recommended medium

Shipping Info

Dry ice

Size

0.5 x 10^6 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What is the source and cell count?

This vial contains 0.5 x 10^6 (500,000) viable macrophages isolated from the lung tissue of a healthy, pathogen-free CD1 mouse.

What's the best way to culture these primary lung macrophages?

We provide a comprehensive, optimized protocol with the cells. They should be thawed quickly in a 37°C bath, gently resuspended in our recommended complete medium, and allowed to adhere and recover before you apply any experimental treatment.

Are these cells suitable for immunology studies?

Yes, they are highly suitable for studies where you need to assess a general, robust immune response rather than a specific Th1 (B6) or Th2 (BALB/c) biased response. They are a common and cost-effective choice for initial pharmacology and toxicology screening.

Viability was high, and they responded consistently to our test compounds.
We use CD1 mice for our general toxicology screens, so these lung macrophages are perfect for our in vitro follow-ups. The quality was excellent, and the cells were very active and highly phagocytic.
It's great to have an outbred option for primary cells
A solid, general-purpose lung macrophage. The 500k count is a bit small but fine for 24-well or 48-well plates. Thawed well and adhered quickly.
Good cells for the price
Exactly as described. CD1 lung macrophages. Clean, viable, and shipped fast. Creative Biolabs is a reliable vendor for primary cells.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.