Leptomeningeal Macrophages, Central Nervous System (MT-0224-HM2)

The cells are obtained from carefully dissected leptomeningeal regions using procedures designed to enrich for border-associated macrophage populations. After isolation, the cells are cultured under conditions supportive of their survival and function while preserving key identity markers. We validate identity by assessing the expression of macrophage markers and, where appropriate, markers associated with border-associated macrophage phenotypes. Morphology and basic functional properties, such as phagocytic capacity, are also evaluated. This multifaceted validation helps ensure that the cells retain core characteristics relevant to their in vivo counterparts.

Yes, leptomeningeal macrophages are particularly well suited for modeling processes that take place at or near CNS barriers. They can be incorporated into BBB or meningeal-like in vitro systems to explore how resident macrophages modulate endothelial behavior, respond to inflammatory cues, or influence the recruitment of peripheral immune cells.

They can be effectively co-cultured with endothelial cells, pericytes, or epithelial cells in transwell systems or microfluidic devices designed to mimic CNS barrier structures. When setting up these co-cultures, carefully consider the medium composition and shear stress (if any), as well as the timing of macrophage addition.