With lower cell counts, efficiency is everything: tight plate layouts, minimized dead volume, and a two-stage plan. We recommend reserving a fraction for baseline QC (viability + phenotype), then running high-value conditions rather than broad, low-replicate matrices. If you share your design, we can help you prioritize conditions to fit the available cells.
Many labs use electroporation of sgRNA (or sgRNA:RNP strategies, depending on design), viral delivery for longer expression windows, or lipid-based transfection for certain formats. The right choice depends on your viability tolerance, desired editing efficiency, and whether you will differentiate into macrophages before readout. We can recommend an approach aligned to your endpoints and available equipment.
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