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Human PB CD14+ Monocytes (Age: 42), 5 x 10^7 cells (MTS-1022-JF36)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What are the recommended storage and handling requirements to protect this larger vial?

The storage requirements are the same as smaller formats: immediate transfer to liquid nitrogen upon receipt and avoidance of repeated freeze-thaw. For larger vials, planning is even more important-prepare media, plates, and counting reagents in advance so the cells spend minimal time in transitional temperatures. If you need partial usage, we recommend designing the experiment to consume the entire vial in one controlled run rather than attempting refreezing.

We need high cell input for multi-omics after stimulation. Is 5 × 10^7/vial suitable for that scale?

Yes. 5 × 10^7 cells per vial is specifically advantageous when your design requires multiple timepoints, replicate conditions, or high-input workflows (e.g., RNA extraction after stimulation, parallel secretome profiling, or multi-panel flow). The larger format helps you keep conditions within the same donor lot to reduce biological variance.

Can you support customized experimental planning (e.g., recommended seeding density, differentiation schedule)?

Absolutely. Many clients use a standard monocyte-to-macrophage workflow but adjust seeding based on plate format, stimulation duration, and endpoint sensitivity. If you tell us your plate type (6/12/24/96-well), the macrophage phenotype target, and readouts (ELISA, flow, imaging, RNA-seq), we can suggest a practical seeding and timeline plan to optimize yield and signal-to-noise-without over-consuming cells.

This format is excellent when you need scale without compromising traceability
The larger vial helped us keep all conditions within the same donor source, which reduced variability across replicates. Post-thaw recovery was stable, and differentiation proceeded predictably.
A strong choice for complex experimental designs
Our project required macrophage generation for multiple co-culture geometries. The vial allowed us to run the full matrix of conditions in one experiment window. We saw consistent adherence and morphology in differentiated macrophages and a clear response to polarization cues.
For teams building multi-endpoint workflows, the larger format is a practical efficiency gain
This product helped us accelerate assay development because it removed one major uncertainty: starting cell input. We used the vial across multiple readouts-flow phenotyping, cytokine panels, and functional uptake assays. Results were coherent across platforms, suggesting the cells were robust and handled stress reasonably well.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.