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Rat Bone Marrow Monocytes (with Cas9 Expression), 0.5 x 10^6 cells (MTS-1022-JF96)

Datasheet

Overview

Description

With Cas9 expression, this product is isolated from the bone marrow of pathogen-free laboratory rat.
0.5 x 10^6 cells are delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Rat

Product Type

Immune cells

Specification

Source

Bone marrow

Format

Cryopreserved

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

0.5 x 10^6 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Related Products

How should I confirm editing success without over-consuming my limited cell number (0.5 million)?

We recommend a two-tier verification approach: (1) a small aliquot for rapid genotyping (amplicon-based assays) or protein-level checks where applicable, and (2) functional confirmation in macrophage endpoints (stimulus response, uptake, cytokines). Because the vial size is modest, we can also advise on minimizing handling loss (gentle centrifugation, optimized recovery time) to preserve cells for your primary readout.

What are the key receiving and storage requirements to protect Cas9-related performance?

Treat these exactly like high-value engineered immune cells: upon receipt, transfer from dry ice to liquid nitrogen for long-term storage, and avoid repeated freeze-thaw. This protects overall cell health, which is essential for consistent editing and differentiation behavior.

Can these cells support co-culture or target-engagement assays after editing?

Yes. After your guide delivery and recovery, these monocytes can be differentiated and placed into co-culture or mechanism-of-action assays (e.g., macrophage-tumor, macrophage-T cell, or pathogen interaction settings). For best interpretability, we recommend incorporating matched controls (non-targeting guide, mock delivery) and tracking baseline activation markers post-delivery to avoid confounding innate activation.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.