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Human PB Pan Monocytes (Age: 30), Negative selected, 5 x 10^7 cells (MTS-1022-JF80)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. Monocytes provide an ideal model for the study of macrophage biology and mechanisms.
These pan monocytes are purified from peripheral blood by removing other cell types using immunomagnetic negative selection, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Isolation Method

Negative selection

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What method is used to enrich the pan monocyte population?

The product is purified from peripheral blood using immunomagnetic negative selection (depletion of other cell types). This approach is widely used when you want enriched cells while minimizing direct antibody capture of the target subset.

What identity and enrichment QC is performed?

The cells are characterized by flow cytometry to support enrichment confirmation (the page references enrichment checks tied to CD14+ gating). This is helpful for aligning to your internal release criteria.

For macrophage differentiation, what is the practical advantage of starting with 5×10^7 pan monocytes in one vial?

The large input cell number supports multi-arm differentiation designs (e.g., multiple cytokine cocktails, time-course harvests, replicate-heavy conditions) without forcing you to mix vials from different lots, which can introduce variability.

The age-30 negative-selected pan monocyte product fit well into our standardized workflow
The large cell quantity supported parallel assay development, training, and internal controls-all from a single lot. Creative Biolabs provided a professional ordering and delivery experience that reduced administrative overhead and helped the science team stay focused on execution.
We used these monocytes as the starting point for macrophage generation in a screening pipeline
The cells differentiated reliably and produced consistent phenotype profiles, which helped us compare treatment conditions with confidence. Baseline activation remained low, and functional responses were robust when stimulated. The 5 × 10^7 scale allowed us to run multiple iterations without worrying about shortages or mid-study vendor switches.
The documentation was thorough enough to satisfy internal QA and to support reporting
Our lab needed a donor-defined monocyte source with sufficient cell numbers to run repeated experiments over several weeks. These age-30 pan monocytes delivered consistent performance-steady recovery, minimal clumping, and clear functional response upon stimulation. The high cell count reduced supply risk and helped maintain continuity in our dataset.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.