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Human PB CD14+ Monocytes (Age: 60), Negative selected, 1 x 10^7 cells (MTS-1022-JF52)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood by removing other cell types using immunomagnetic negative selection, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
1 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Isolation Method

Negative selection

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

1 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

This vial is 1×10^7 cells. Who is it best suited for?

A 1×10^7 vial is ideal for pilot studies, method development, single-condition proof-of-concept experiments, or smaller co-culture setups where you want to avoid unused excess cells. It lets you validate stimulation conditions, differentiation timing, or a flow gating strategy before scaling up to larger cell numbers.

Can I split one vial across multiple experiments?

You can split the contents after thaw into multiple assay arms on the same day, but do not refreeze leftover cells. The page explicitly advises against repeated freeze-thaw cycles because they can reduce viability and shift functional phenotypes. Plan a single-day workflow that uses the full vial across replicates/conditions.

What donor screening is performed, and is this "normal" donor material?

Donors are screened for HBV, HCV, HIV-1/2, WNV, and CMV, and the product disease state is listed as normal. This makes it appropriate as a baseline starting material for mechanistic studies and assay development where you want controlled donor status.

The 1×10^7 negative-selected CD14+ monocytes were ideal for our pilot study where we needed smaller scale without sacrificing quality
Thawing was straightforward, recovery was strong, and the low level of contaminating lymphocytes made downstream phenotyping much cleaner. We ran a short M-CSF differentiation followed by polarization and saw crisp marker separation with minimal background. The smaller vial size also helped us avoid waste and budget overruns.
Shipping conditions were well controlled, and the product arrived with professional labeling
These negative-selected monocytes performed very well-post-thaw recovery allowed us to proceed with differentiation quickly, and the resulting macrophages showed stable expression profiles in our controls. The reduction in contaminating cell types helped us attribute signals more confidently to the monocyte/macrophage lineage.
We tested this product in a microfluidic culture format where cell stress can be amplified
The monocytes tolerated the transition nicely: they adhered, differentiated, and remained viable in the device environment. Negative selection appeared to reduce unwanted activation and improved the clarity of our functional readouts. The small vial size was convenient for iterative optimization without committing to large quantities upfront.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.