Online Inquiry

Human PB CD14+ Monocytes (Age: 52), 5 x 10^7 cells (MTS-1022-JF44)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Can we use these monocytes to generate macrophages for long-term assays (7-14 days)?

Yes, with the right differentiation conditions and feeding strategy. For longer cultures, consistency in cytokine supplementation, media refresh cadence, and seeding density becomes critical. We also recommend monitoring morphology and marker expression at defined checkpoints to ensure the phenotype remains aligned to your study goal rather than drifting due to over-confluence or nutrient stress.

What viability should we expect after thaw, and what factors most influence it?

Post-thaw viability can vary by donor and handling, but the biggest controllable factors are thaw speed, DMSO exposure time, centrifugation conditions, and whether the cells experience temperature fluctuations. We provide handling guidance to help you protect viability and functional responsiveness. If your assay is especially sensitive, we can advise on a short recovery/rest step before differentiation.

Do you support pairing these cells with matched plasma or PBMC fractions for more physiologic immune modeling?

Depending on availability and your study design, we can discuss options to align monocyte-derived macrophages with complementary immune components to build more physiologically relevant systems. If your goal is immune cross-talk modeling, we can help you structure a sourcing and experimental plan that maintains donor consistency where it matters most.

If you're building a robust dataset that needs consistency over time, this lot is well-suited
For our macrophage biology work, baseline stability is just as important as induced responses. This lot delivered both. Controls remained quiet, while stimulation produced clear cytokine induction and expected transcriptional shifts. We also found the plating behavior consistent, which improved assay uniformity. The larger vial size reduced procurement friction and allowed us to plan confidently across multiple experimental cycles.
We used macrophages derived from this lot in proteomic and transcriptomic profiling
Consistency across replicates was a standout benefit, and the results aligned with expected biological pathways. The ability to keep the full study within one donor lot reduced noise and made downstream analysis cleaner. Operational details-labeling, documentation, and QA notes-were also solid, which matters when multiple departments need to sign off on sample provenance and handling.
This vial size and performance profile make it a strong fit for functional screening
Our team focuses on particle uptake and macrophage functional capacity, and this lot delivered stable, interpretable data. We observed consistent uptake kinetics and clear responses to modulators, with less well-to-well variability than expected. The derived macrophages were robust during repeated washes and imaging steps. When you're running high-throughput uptake assays, dependable primary cells make the difference between a publishable dataset and a troubleshooting marathon.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.