Online Inquiry

Human PB CD14+ Monocytes (Age: 26), Negative selected, 5 x 10^7 cells (MTS-1022-JF15)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood by removing other cell types using immunomagnetic negative selection, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Isolation Method

Negative selection

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Can we use these cells for high-throughput macrophage generation and compound screening?

Yes. The high cell count is well suited for screening pipelines that require multiple plates, timepoints, and conditions. We can help you define a standardized differentiation and stimulation schedule, recommend performance checkpoints, and optionally provide functional validation services. Many screening teams value a predictable, repeatable workflow that minimizes operator variability, and we can align deliverables to support that operational need.

How do you recommend storing and managing inventory for a large lot?

Define an inventory map so vials are retrieved in a controlled sequence, store them in stable long-term conditions, and avoid unnecessary exposure during transfers. If you plan multiple campaigns, allocate vials to specific campaigns in advance and keep a record of retrieval dates and storage locations. This operational discipline helps maintain consistency across time and reduces the risk of accidental temperature excursions.

Can you support custom assay development beyond the cells themselves?

Yes. We can support assay design and execution around macrophage differentiation, polarization, and functional readouts. If your end goal is a decision-ready data package, we can align on acceptance criteria, standardized reporting, and a workflow that reduces iterations. This is particularly useful for teams building new macrophage-based screening assays.

The ordering process was straightforward, and support was responsive when we asked for recommendations on rest time and seeding density
We needed donor-age-defined monocytes for a standardized panel, and this product delivered reliable performance. The cells were easy to thaw and process, and we appreciated the low clumping and minimal debris. Baseline cytokine output was low, giving us better sensitivity in treatment comparisons. Differentiated macrophages showed strong functional activity and stable viability across our assay window.
Our lab often struggles with monocytes that arrive stressed, but this vial behaved calmly and predictably
Negative selection seemed to preserve functional responsiveness without introducing activation artifacts, which made our downstream cytokine and signaling assays easier to interpret. The macrophage differentiation phase was also smooth, yielding uniform adherence and consistent marker progression. With 5×10^7 cells, we could run multiple assay types in parallel and still keep backup aliquots for repeat runs.
A solid purchase for rigorous mechanistic workflows
The negative-selected monocytes produced clean RNA and consistent protein yields after differentiation, and we saw low ambient noise in baseline conditions. The large cell count supported multiple timepoints and technical replicates, which improved confidence in our analysis. Functionally, the cells responded strongly to TLR ligands while maintaining low background activity.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.