Online Inquiry

Human PB CD14+ Monocytes (Male), 5 x 10^7 cells (MTS-1022-JF59)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

If I'm building a sex-comparison dataset, how does a male donor lot help?

A male-designated lot lets you control donor sex as a variable, which is valuable when comparing innate immune responsiveness, activation thresholds, or differentiation outcomes across male vs female donors. Keeping sex consistent within cohorts improves interpretability and reduces hidden confounding factors.

Is 5×10^7 cells/vial appropriate for co-culture and multi-condition assays?

Yes. A 5×10^7 vial supports multi-plate designs (e.g., co-culture ratios, multiple stimuli, time points, and replicates) while keeping all conditions within one donor lot-especially helpful when data comparability is a priority.

What is the single most important "do not do" after we receive the shipment?

Do not allow the vial to warm up or undergo repeated freeze-thaw cycles. The handling notes emphasize immediate transfer to liquid nitrogen storage and warn that repeated freezing/thawing is not recommended because it can reduce viability and affect downstream performance.

The QC documentation was concise yet useful
This male-donor CD14+ prep differentiated uniformly and produced consistent cell density and morphology across plates. That directly improved our image segmentation and reduced analysis artifacts. The 5×10^7 format also helped us keep conditions consistent across experiments without frequent re-ordering.
This is a research-ready product that supports both scientific quality and efficient project execution
Our lab needed male-donor monocytes for a sex-stratified macrophage project, and this product helped us execute without operational headaches. The cells arrived in strong condition, recovered quickly, and behaved predictably through differentiation and stimulation. Replicate variability was low, which improved our statistical power and reduced the number of repeat plates.
The cells differentiated smoothly and gave clean cytokine readouts with lower background than we typically see with mixed PBMC isolations
The high-yield vial supported multiple conditions and time points, which was key for our study design. The ordering and shipping process was efficient, and documentation supported internal traceability.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.