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Human PB Pan Monocytes (Age: 35), Negative selected, 5 x 10^7 cells (MTS-1022-JF81)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. Monocytes provide an ideal model for the study of macrophage biology and mechanisms.
These pan monocytes are purified from peripheral blood by removing other cell types using immunomagnetic negative selection, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Isolation Method

Negative selection

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

I'm doing immune-interaction assays (e.g., co-culture). Why might pan monocytes be a better starting point than a single-marker-enriched subset?

For interaction assays, a broader monocyte compartment can sometimes provide a more representative starting population for differentiation and functional readouts-especially if your objective is to capture population-level responses rather than one narrowly defined subset. This listing is positioned for macrophage biology and mechanism studies.

What does negative selection change for downstream activation-sensitive assays?

Negative selection enriches monocytes by removing other cell types, which many researchers prefer when they want to reduce manipulation artifacts before stimulation (e.g., early cytokine release, receptor-ligand response testing, polarization sensitivity).

What vial dose do I receive and how does that impact replicate design?

You receive 5×10^7 cells per vial, which supports dense replicate matrices and multi-endpoint sampling (phenotype + cytokines + transcriptomics) from a single vial source.

This monocyte lot served as the foundation for a macrophage production run supporting both phenotyping and downstream functional studies
The scale supported multiple plates, time points, and internal controls from a single order. Documentation was clear and organized, which saved time for our operations team. We appreciated the end-to-end reliability-from ordering to delivery to performance-and we will continue using donor-defined, negative-selected formats for large programs.
This is a well-suited product for groups building standardized immune assay pipelines under tight timelines
For an internal method validation, we needed a high-volume monocyte supply that would behave predictably for repeated runs. These age-35 negative-selected pan monocytes provided consistent performance across multiple batches of experiments. Handling was straightforward and the cells showed minimal clumping after thaw. The functional response upon stimulation was robust and reproducible, enabling clear benchmarking of assay sensitivity.
We used this 5 × 10^7 age-35 monocyte lot for macrophage differentiation followed by functional stimulation and marker profiling
The cells seeded evenly and differentiated consistently across plates. Baseline activation remained controlled, which improved interpretability of early signaling and cytokine measurements. The donor information was clearly documented, supporting our cohort design.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.