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Human PB CD14+ Monocytes (Age: 28), Positive selected, 1 vial (MTS-1022-JF21)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood using immunomagnetic positive selection directed against CD14, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Isolation Method

Positive selection

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

1 vial

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

How do you recommend validating the cells quickly before committing them to a multi-day differentiation protocol?

Many teams perform a rapid post-thaw viability check and a brief flow confirmation panel for identity/purity. We also recommend a small "pilot seeding" portion to confirm adherence behavior and baseline morphology before scaling the rest into the full differentiation schedule.

Can I use this vial to generate macrophages for both functional assays and imaging-based endpoints?

Yes. Positive-selected monocytes often differentiate predictably and work well for functional readouts (e.g., uptake/phagocytosis-style assays) and imaging endpoints when standardized seeding and media schedules are followed. We can advise on density and timing to help your images remain comparable across replicates.

What if I need the same donor age and selection method again for follow-up studies?

We can help you plan a continuity strategy, including recommended ordering windows and lot planning. For follow-up programs, we can also advise on assay design controls to ensure your comparisons remain valid across time.

This product supported a full workflow for us: quick identity check, differentiation, and then a functional assay panel
The positive selection enrichment helped our readouts look cleaner-less background from non-target populations and more consistent macrophage markers after differentiation. We also liked that the supplier shared practical guidance on a short rest period to stabilize baseline before stimulation. The vial arrived in excellent condition, and post-thaw recovery was strong.
It fit our workflow well and produced stable, interpretable results for RUO applications
We used these monocytes for imaging-heavy experiments where cell behavior and morphology need to be consistent across repeats. The positive-selected cells adhered and transitioned predictably during differentiation, and our imaging endpoints were comparable across plates. That consistency reduced the time we usually spend troubleshooting variability.
The overall experience-from ordering to experimental outcomes-was smooth and professional
We ordered this vial to support a pilot study and ended up using it as our reference material because the performance was so consistent. Post-thaw recovery was solid, the cells were responsive, and our differentiation protocol produced expected phenotypes on schedule. The positive selection enrichment contributed to clean downstream signals in our assays.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.