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Human PB CD14+ Monocytes (Age: 53), Negative selected, 5 x 10^7 cells (MTS-1022-JF45)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood by removing other cell types using immunomagnetic negative selection, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Isolation Method

Negative selection

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

How do you ensure these negatively selected monocytes are not pre-activated?

We standardize processing and avoid unnecessary stressors during isolation and cryopreservation. For activation-sensitive programs, we recommend adding baseline checks (e.g., select activation markers) and running a small pilot stimulation to confirm responsiveness without elevated background. This gives you a data-backed confidence layer before you commit to larger experiments.

What controls should we include to interpret donor variability in negative-selection lots?

We recommend consistent recovery timing, unstimulated controls, and-when possible-benchmark stimuli that provide a reference response window. Including a small panel of phenotyping markers at baseline can also help you contextualize donor-to-donor differences and reduce the risk of over-interpreting biological noise as treatment effect.

Excellent operational reliability for long runs
We ran this lot across several weeks of experiments, including staggered differentiation batches. Performance remained consistent: strong recovery, stable plating, and predictable differentiation outcomes. The product guidance emphasized avoiding repeated freeze-thaw, which matched our handling plan and helped maintain quality.
Clean macrophage phenotype separation in polarization models
Macrophages derived from this negative-selected lot showed strong, consistent separation in marker expression and cytokine profiles. We also appreciated the low background noise in controls, which made intermediate phenotypes easier to interpret. The vial size supported full replication and rescue arms, strengthening our experimental design.
We used this lot as the backbone for a multi-assay validation program
Because we stayed within one donor lot, we reduced variability and improved internal confidence in cross-assay comparisons. The negative-selected enrichment aligned with our receptor-focused work, and the macrophages held up well during handling-heavy protocols. If you need one lot to support a broad validation package, this product provides both the scale and the functional stability to do it.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.