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Human PB CD14+ Monocytes (Age: 50), Negative selected, 2.5 x 10^7 cells (MTS-1022-JF43)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood by removing other cell types using immunomagnetic negative selection, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
2.5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Isolation Method

Negative selection

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

2.5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Does negative selection change purity or yield compared to positive selection?

Both methods can produce high-quality monocyte preparations, but they optimize different priorities. Negative selection is often favored for preserving a more "untouched" phenotype, while positive selection can be highly efficient for capturing CD14+ cells. The best choice depends on your downstream assay sensitivity, required purity threshold, and acceptable yield. We can recommend the most suitable approach based on your endpoints.

How do you verify that the isolated population remains functionally responsive after negative selection?

We typically recommend confirming CD14 enrichment by flow cytometry and evaluating post-thaw viability, then using a lightweight functional assay aligned to your planned biology-such as a standardized stimulation response readout. For projects where subtle signaling differences matter, adding activation-marker baselining can help demonstrate that the population is not inadvertently primed.

What are the key handling precautions specific to negatively selected monocytes?

The same core best practices apply-gentle handling, rapid thaw, quick DMSO removal, and a standardized recovery period. Additionally, because negative selection is often chosen to preserve a resting phenotype, we recommend extra attention to endotoxin control and avoiding unnecessary mechanical stress that can trigger activation-like signatures.

Strong option for macrophage polarization comparisons
We ran M1/M2 polarization side-by-side and saw clear separation in marker profiles and secreted factors. The negative-selected starting material seemed to reduce background and improve interpretability, especially for intermediate phenotypes. The lot also held up operationally: consistent plating, stable viability, and no unusual debris.
Clean performance in co-culture systems
In barrier and co-culture systems, overly activated macrophages can confound outcomes. This lot produced macrophages that remained stable and well-behaved in co-culture, with controlled responses to stimulation rather than constant background inflammation. That stability improved our readouts and reduced the need for heavy normalization.
We used this lot across multiple weeks to support a project timeline with staggered assay runs
The ability to keep the same donor/lot helped reduce variability, and the negative-selected enrichment aligned with our receptor-integrity requirements. The cells differentiated consistently and produced stable functional readouts over time. From a project management perspective, this is the kind of material that reduces surprises and keeps timelines predictable.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.