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Human PB CD14+ Monocytes (Age: 57), Negative selected, 5 x 10^7 cells (MTS-1022-JF50)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood by removing other cell types using immunomagnetic negative selection, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Isolation Method

Negative selection

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

We run multi-plate stimulation studies. Is 5×10^7 cells enough, and how should we plan allocations?

This cell vial is ideal for high-throughput stimulation panels, multiple time points, or parallel phenotyping (flow + RNA + secretome). We recommend planning allocations by (i) expected post-thaw recovery, (ii) seeding density per well format, and (iii) whether you need extra cells for QC or pilot titrations. If you need matched donors across conditions, consider ordering multiple vials from the same lot to minimize variability.

What are the most common handling mistakes that reduce viability or alter phenotype after thaw?

The biggest risks are slow thawing, harsh centrifugation, and repeated freeze-thaw. We recommend rapid thawing, gentle dilution into pre-warmed medium, low-speed spins, and minimizing pipetting stress. Store immediately in liquid nitrogen upon receipt, and avoid re-freezing. These steps help preserve plating efficiency and keep baseline activation low.

Can I use these monocytes to generate macrophages or dendritic cells directly?

Yes. CD14+ monocytes are widely used as precursors for monocyte-derived macrophages (MDMs) and monocyte-derived dendritic cells (moDCs). With a consistent starting population, you can run differentiation then polarization/activation panels and compare phenotypes across treatments. If you have a specific marker panel or endpoint (e.g., phagocytosis, cytokines, antigen presentation), we can help recommend an assay-ready workflow.

Support was responsive and practical, helping us align thawing density and resting time for our specific culture format
We ordered the age-57, negative-selected CD14+ monocytes to standardize our macrophage differentiation workflow across multiple assays. The lot arrived well packaged with clear labeling and an easy-to-follow QC summary. Post-thaw recovery was strong, and the cells behaved consistently during M-CSF differentiation. What impressed us most was the low background from non-monocytic contaminants, which noticeably reduced noise in downstream cytokine readouts.
We chose the negative-selection format because we wanted minimal activation artifacts prior to polarization
The cells arrived in excellent condition and recovered smoothly. In our hands, baseline activation markers stayed low after thaw, which is essential for comparing M1/M2 polarization profiles. The product's consistency helped us reduce the number of repeats and saved weeks of troubleshooting. Communication was another highlight: order confirmation was fast, and the team proactively shared handling tips tailored to our media and seeding density.
This vial set fit perfectly into our SOP for monocyte-derived macrophage assays
The negative-selected CD14+ population gave cleaner differentiation outcomes than our prior magnetic-positive workflows, especially for downstream ELISA and multiplex cytokine panels. We also ran a quick mycoplasma check and morphology assessment after plating-everything looked excellent.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.