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Human PB CD14+ Monocytes (Age: 25), 2.5-5 x 10^7 cells (MTS-1022-JF12)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
2.5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

2.5 x 10^7 cells, 5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

How should we plan experiments with a variable cell count?

When a product is offered in a range, we recommend designing your workflow around the minimum guaranteed count and treating any additional cells as buffer for optimization or extra replicates. The most practical approach is to pre-map your must-have conditions and controls, then allocate optional conditions only if the final post-thaw count supports them.

How do you recommend setting up macrophage differentiation for consistent results?

Consistency hinges on standardized seeding density, a controlled recovery period post-thaw, defined cytokine schedule, and consistent medium changes. Many labs unknowingly introduce variability by changing adherence windows or timing stimulation relative to differentiation day. We provide a practical schedule that aligns to your readouts and can suggest checkpoints (morphology, basic phenotyping) to confirm the culture is on track before committing cells to expensive downstream assays.

Can you provide support for polarization and functional assays if our team wants a turnkey option?

Yes. We can provide add-on services to generate differentiated macrophages (and polarized states if needed) plus standardized validation readouts. This reduces operator-to-operator variability and can accelerate programs that are running on tight timelines or that require consistent data packages for clients or internal go/no-go decisions.

It delivered the consistency we need for comparative datasets and saved us the time of in-house enrichment
This age-25 CD14+ monocyte vial was ideal for our mid-scale study design. The cell number let us run multiple stimulation conditions, timepoints, and replicates without compromising statistical power. The thaw was clean, viability was strong, and we saw minimal clumping, which reduced cell loss during washes. Differentiation into macrophages proceeded smoothly, producing uniform cultures that responded predictably to polarization cues.
We purchased this prep for a co-culture project with tumor spheroids
The monocytes recovered well and differentiated into macrophages with uniform adherence and stable viability over several days. In functional readouts, the macrophages showed consistent phagocytosis and cytokine secretion profiles, and we were able to reproduce results across plates.
The 2.5-5×10^7 format was a sweet spot for us
We used the monocytes both for immediate stimulation assays and for macrophage differentiation followed by RNA-seq. Both applications worked well-good recovery, low baseline activation, and strong responses to stimuli. The cell prep was easy to handle, and the guidance on thawing and resting reduced stress artifacts.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.