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Human PB Monocytes, Negative selected, 1 vial (MTS-1022-JF77)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood by removing other cell types using immunomagnetic negative selection.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Isolation Method

Negative selection

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

1 vial

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What does "1 vial" mean for experimental planning-do I get a fixed cell count?

This SKU is defined as 1 vial (cryopreserved, negative selection). For projects where the unit of use is "per vial" (single run, pilot test, or discrete batch qualification), this format simplifies ordering and inventory control. If you need a strict cell-number-per-vial specification for plate mapping, we can align you to the best-matched fixed-count SKU.

How are these PB monocytes enriched in the negative-selected version?

They are prepared by immunomagnetic negative selection, i.e., removing other cell types to enrich monocytes, and the enriched population is characterized by flow cytometry for CD14+ enrichment confirmation.

What is the correct intake workflow when the vial arrives?

It ships on dry ice. Upon receiving, transfer immediately to liquid nitrogen (-150°C to -190°C) and avoid repeated freeze-thaw. The handling note explicitly states viability cannot be guaranteed if the procedure is not followed.

The vial format is ideal for our lab because we often run targeted experiments that don't require large cell counts
These negative-selected monocytes arrived in good condition and performed consistently across replicate plates. We used them in a short-term stimulation assay and obtained clean, interpretable results with low baseline cytokine release. The ordering process was smooth, and shipment status updates were timely.
The small format allowed us to run optimization experiments without committing to large lots
We were testing a macrophage differentiation workflow and needed monocytes that would not introduce background noise. This negative-selected vial performed nicely: consistent recovery, minimal clumping, and stable baseline phenotype. After differentiation, the macrophages displayed expected morphology and responded robustly to polarization cues.
This 1-vial negative-selected monocyte product was used to confirm a new stimulation protocol before scaling up
The cells handled well, showed minimal spontaneous activation, and generated reproducible response curves across concentrations. The vial size reduced waste and helped us iterate quickly without unnecessary inventory.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.