Online Inquiry

Human PB CD14+ Monocytes (Age: 27), Positive selected, 1 vial (MTS-1022-JF18)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood using immunomagnetic positive selection directed against CD14, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Isolation Method

Positive selection

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

1 vial

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

How should we design a pilot experiment to maximize what we learn from a single vial?

A high-value pilot typically includes: (1) baseline recovery and minimal phenotype confirmation, (2) a small matrix of density and cytokine conditions to confirm differentiation behavior, and (3) one or two stimulation conditions aligned to your primary readout. Keep the design tight-avoid too many conditions with insufficient replicates.

What downstream services can you provide if we want to outsource part of the workflow?

We can support monocyte-to-macrophage differentiation, polarization workflows, and functional validation assays with structured deliverables. This is valuable when your internal team needs to reduce variability, accelerate timelines, or generate a standardized data package. We can align deliverables to your endpoints and reporting needs so the output is immediately usable for decision-making.

What support do you provide after delivery if we encounter unexpected results?

We provide practical troubleshooting focused on workflow variables: thaw technique, medium composition, density, timing, and stimulation strategy. We'll help you identify the most likely root cause and propose a minimal set of changes to recover performance without burning through the sample.

We needed a ready-to-use CD14+ monocyte vial with minimal variability
The cells were easy to thaw and process, and the population appeared well enriched. Macrophage differentiation was consistent, and downstream assays produced strong dynamic ranges with low baseline background. The vendor's instructions were pragmatic and helped reduce cell loss during washes.
Reliable performance like this makes primary-cell experiments much easier to manage and interpret
We used this vial as a control donor in a multi-batch study and found it highly consistent. Thawing and recovery were smooth, and the monocytes tolerated handling well. Differentiated macrophages showed stable viability and strong functional activity, including phagocytosis and cytokine induction. The positive-selected prep helped us reduce variability and streamline our workflow, especially when multiple operators are involved.
This positive-selected vial worked very well for our standardized macrophage polarization panel
We saw strong post-thaw recovery and minimal debris, and the cells differentiated uniformly in M-CSF. The resulting macrophages showed clear phenotype shifts under IFN-γ/LPS versus IL-4/IL-13 conditions, with consistent marker profiles across wells. That level of uniformity is valuable when you're comparing treatment effects and need tight replicate variance. Communication was also smooth when we asked a technical question about recommended seeding density.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.