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Rat Bone Marrow Monocytes (with GFP Expression), 0.5 x 10^6 cells (MTS-1022-JF97)

Datasheet

Overview

Description

With GFP expression, this product is isolated from the bone marrow of pathogen-free laboratory rat.
0.5 x 10^6 cells are delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Rat

Product Type

Immune cells

Specification

Source

Bone marrow

Format

Cryopreserved

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

0.5 x 10^6 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Is GFP helpful for macrophage differentiation workflows or only for imaging?

It supports both. Imaging is the obvious benefit, but GFP is also valuable for cell recovery and gating during differentiation (e.g., distinguishing your macrophage lineage from other populations in mixed systems). To keep signals clean, we recommend aligning filter sets/compensation early and defining a consistent gating template before scaling experiments.

What handling steps are most important to maximize recovery from a 0.5 million-cell vial?

Use gentle handling: rapid thaw, gradual dilution, minimal centrifugation force, and immediate placement into optimized culture conditions. The page explicitly warns against repeated freeze-thaw and instructs liquid-nitrogen storage upon receipt-those steps matter directly for recovery.

Can GFP intensity drift after differentiation or stimulation?

Fluorescent intensity can vary with activation state, culture stress, and imaging settings. To protect interpretability, design your analysis around relative changes (within-run controls) and consider reporting both percent GFP+ and median fluorescence intensity (MFI) where relevant. If you share your planned endpoints, we can suggest an acquisition/analysis strategy that fits your platform.

We purchased GFP-expressing rat monocytes to validate a macrophage differentiation protocol and monitor lineage progression in real time
The label made it easy to follow cells through the recovery stage, attachment, and early maturation, reducing uncertainty during protocol optimization. The cells showed uniform morphology and good survival, and the fluorescence did not interfere with downstream flow cytometry staining panels. The included QC and handling guidance helped us hit the ground running.
Shipping was reliable and the technical notes were practical
Our team runs microfluidic migration assays where cell identification is critical. These GFP monocytes enabled straightforward tracking in narrow channels and complex gradients without additional staining. The cells retained typical monocyte-like behavior and responded to chemotactic cues predictably. We were able to quantify motility parameters with confidence, thanks to clean fluorescence and low background.
The product documentation was clear, and the behavior matched what we expect from true primary monocytes
The GFP signal was bright and uniform enough for both microscopy and flow-based gating, which made population tracking far more reliable than staining approaches. The cells recovered well and maintained fluorescence across the early culture window. This helped us quantify attachment efficiency and monitor morphological changes without extra dyes that can stress primary cells.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.