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Human PB CD14+ Monocytes (Age: 69), 2.5 x 10^7 cells (MTS-1022-JF57)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
2.5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

2.5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Older-donor monocytes can be fragile. What should we do to maximize recovery after thaw?

Focus on gentle handling: rapid thaw, immediate dilution into warm medium, minimize pipetting, and avoid harsh spins. Use a short recovery period before strong stimulation. Most importantly, do not refreeze leftovers-repeat freeze-thaw is explicitly discouraged and can disproportionately affect sensitive primary cells.

What is the exact vial size and format for this age-69 lot?

This listing is 2.5×10^7 cells per vial, provided cryopreserved and shipped on dry ice, supporting standardized scheduling for experiments and long-term storage in liquid nitrogen.

Can you advise how to structure an immunosenescence-focused study using this lot?

A practical approach is to pair the age-69 lot with younger-age lots under identical thaw, seeding, and stimulation conditions, and include baseline (unstimulated) controls to capture intrinsic donor tone. If you share your endpoints (cytokines, transcriptomics, phagocytosis, antigen presentation), we can recommend an allocation strategy that preserves enough cells for both validation and final runs.

This product performed well in a set of assays focused on innate immune responsiveness
After thaw, the cells stabilized quickly, and baseline activation remained manageable after a short rest. We observed clear response patterns upon stimulation, and the results were consistent across replicate wells.
We appreciated how "ready for workflow" this preparation felt
The vial quantity matched our plate plan, and the cells performed consistently during differentiation and stimulation. We saw lower background in cytokine assays compared with mixed PBMC-derived starts, which improved interpretability. The order process was smooth, and the team was responsive when we asked about recommended seeding densities.
The age-69 CD14+ monocytes delivered predictable recovery and consistent differentiation
We saw strong macrophage marker progression, and the cells tolerated routine media changes. The product's performance helped our study stay on schedule, and the documentation made auditing easier.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.