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Human PB CD14+ Monocytes (Age: 41), Positive selected, 1 vial (MTS-1022-JF34)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood using immunomagnetic positive selection directed against CD14, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Isolation Method

Positive selection

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

1 vial

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

How should we handle the shipment upon arrival to protect viability and plating efficiency?

These cells are shipped on dry ice and should be transferred immediately to vapor-phase liquid nitrogen (approximately -150°C to -190°C) for storage. The most common avoidable cause of viability loss is temperature fluctuation during intake-so we recommend preparing LN2 storage in advance and minimizing time at ambient conditions. Also, do not aliquot and refreeze; repeated freeze-thaw cycles can compromise recovery and experimental reproducibility.

Can I use these cells directly for phenotyping, or do I need a rest period after thawing?

For most workflows, we recommend a post-thaw recovery period (often overnight) before sensitive functional assays. You may perform basic viability assessment and surface-marker checks shortly after thaw, but if you're evaluating activation markers, cytokine release, or polarization, a short recovery helps reduce thaw-induced stress signals. If you share your target readouts (e.g., M1/M2 polarization markers, phagocytosis, antigen presentation), we can suggest a thaw-to-assay timeline aligned with those endpoints.

Solid product for research-grade macrophage work
We ordered the Age 41 PB CD14+ monocytes (positive selection) to seed a macrophage differentiation workflow used for screening immunomodulatory candidates. The vial arrived on dry ice in excellent condition, and recovery after thaw was strong with consistent adherence and morphology in our differentiation medium.
The team's responsiveness during ordering and their storage guidance (LN2 transfer immediately, avoid repeated freeze-thaw) likely saved us from common mistakes
This product helped us standardize a training protocol for new lab members who were struggling with inconsistent monocyte inputs. The positive-selection CD14+ prep was straightforward to thaw and plate, and we achieved reproducible macrophage yields across two separate runs.
I'd use it again for mechanistic macrophage studies
As a CRO, we need suppliers that minimize experimental risk and reduce troubleshooting time. This product integrated smoothly into our cytokine profiling and phagocytosis assays without introducing unexpected variability.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.