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Human PB Pan Monocytes (Age: 22), Negative selected, 5 x 10^7 cells (MTS-1022-JF79)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. Monocytes provide an ideal model for the study of macrophage biology and mechanisms.
These pan monocytes are purified from peripheral blood by removing other cell types using immunomagnetic negative selection, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Isolation Method

Negative selection

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What is the isolation method and how does it help preserve functional behavior?

This product is prepared by immunomagnetic negative selection (removing other cell types), which is commonly chosen when teams want enriched monocytes without direct target capture that may bias receptor status before stimulation.

I'm running a large study-what is the vial dose here and how does it help throughput?

The vial contains 5×10^7 cells, which is well-suited for high-throughput plans such as multi-condition polarization matrices, co-culture panels, repeated sampling for cytokines, and parallel RNA/protein extractions.

What safety/QC assurances do I get for primary human sourcing?

Donors are screened for HBV, HCV, HIV-1/2, WNV, and CMV, and the cells are reported negative for bacteria, yeast, fungi, and mycoplasma-supporting more stable culture outcomes.

We used these age-22 pan monocytes to build a robust macrophage production pipeline for downstream functional assays
The negative-selection approach helped preserve a calm baseline state, which was important for our sensitive readouts. The cells handled well, with limited clumping and consistent seeding performance. Across multiple plates, the differentiated macrophages showed uniform morphology and consistent response to polarization cues.
The high cell number reduced operational stress and minimized the need for staggered orders
For a project with tight deadlines, we needed a large monocyte input that would behave consistently across batches of experiments. This age-22 negative-selected pan monocyte product arrived on schedule and performed reliably in culture.
The 5 × 10^7 format was exactly what we needed for a multi-assay campaign-polarization, cytokine profiling, and transcript-level follow-ups
These age-22 pan monocytes recovered well after thawing and showed minimal spontaneous activation, which improved our baseline stability. We achieved consistent differentiation outcomes across different operators, and the functional responses were robust and reproducible. The order experience was smooth, and the documentation was complete enough for our internal approvals.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.