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Monocyte Differentiation Media (MTS-1223-HM15)

Datasheet

Overview

Description

Creative Biolabs offers monocyte differentiation media for differentiation of human pluripotent stem cells (hPSCs) to monocytes.

Applications

Cell Culture, Differentiation, Expansion

Product Type

Media

Specification

Size

1 Kit

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What is this kit designed for-primary monocytes or stem-cell differentiation?

This product is specifically positioned as monocyte differentiation media for differentiating human pluripotent stem cells (hPSCs) to monocytes, supplied as 1 kit, and intended for cell culture/differentiation/expansion workflows.

What should I measure to confirm I truly generated monocytes from hPSCs?

Confirmation is usually multi-layered: morphology, core monocyte markers by flow cytometry, and functional checks (e.g., differentiation potential toward macrophage phenotypes, baseline innate responsiveness). If you share your platform (markers/instruments), we can propose a lean "release criteria" panel that is publishable and scalable.

How do I plan production if I need monocytes repeatedly across a long development timeline?

Treat it like a production pipeline: define a master hPSC maintenance SOP, lock your differentiation schedule, and keep a reference batch for comparability. For long programs, we recommend building in periodic benchmarking (marker expression + 1-2 functional assays) so you can prove continuity across months, not just within a single run.

This differentiation medium helped our team quickly establish a reliable monocyte-to-macrophage pipeline for client projects
The resulting cells showed consistent behavior across functional assays, and the differentiation process was straightforward with minimal batch-to-batch surprises. We also found that the medium supported downstream molecular assays, including RNA extraction, without obvious stress artifacts. The overall workflow became more predictable, which is essential in a CRO setting where timelines matter.
The medium reduced protocol "tuning" time and allowed faster onboarding of new staff
We used this differentiation medium in an imaging-centric workflow, and it improved both morphology uniformity and assay readiness. Differentiated cells adhered well and showed consistent spreading, which made segmentation and quantification far easier. The cells also tolerated stimulation and wash steps without dramatic detachment, improving assay robustness.
It's an efficient way to get reliable monocyte-derived macrophages without over-optimizing every batch
Our lab focuses on macrophage functional phenotyping, and the differentiation step is critical for clean baseline behavior. With this medium, the cells matured in a predictable timeframe and produced consistent marker profiles by flow cytometry. We appreciated that the differentiated cells were robust enough for routine handling and assay setup.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.