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Human Cord Blood CD14+ Monocytes, Positive selected, 1-5 x 10^7 cells (MTS-1022-JF7)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.

These human CD14+ monocytes are purified from peripheral blood using immunomagnetic positive selection directed against CD14, with the highest viability and plating efficiency.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
1 x 10^7 cells per vial.

Species

Human

Product Type

Immune cells

Specification

Source

Bone marrow

Isolation Method

Positive selection

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

1 x 10^7 cells, 5 x 10^6 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

We're running macrophage differentiation and polarization-what are the most critical handling steps to preserve function and avoid unnecessary activation?

For primary monocytes, the biggest performance drivers are process variables: thaw speed, wash intensity, seeding density, recovery time, and stimulation timing. To keep baselines stable, lock plate type, density, media formulation, and the exact day/time you apply differentiation cytokines and polarization stimuli.

We plan to use the 1.5 × 10^7 cells lot for multi-condition work-how do you recommend allocating cells across conditions, timepoints, and assay types?

Start by reserving a small fraction for baseline checks (viability and a minimal phenotype panel) so you can confirm the input quality before committing to expensive assays. Next, allocate cells to your highest-value conditions with sufficient replicates, and keep timepoints tightly tied to your mechanistic question. If you have both functional and molecular endpoints, a staged strategy often works best: validate differentiation/polarization performance first, then dedicate remaining material to RNA/proteomics or larger panels.

If post-thaw recovery or downstream functional performance is lower than expected, what support do you provide and what are the first corrective steps?

We support rapid, experiment-oriented troubleshooting. In most cases, performance issues trace to controllable variables: over-washing, harsh centrifugation, inconsistent density, insufficient rest time, or stimulation applied too early/late relative to differentiation. We'll review your steps and endpoints, then recommend targeted adjustments.

We ran viability and mycoplasma checks upon receipt and everything aligned with expectations
The cells tolerated a short rest period post-thaw and then performed well in a monocyte-derived dendritic cell differentiation pilot, which is not always forgiving. The vendor's documentation and packing slip details were clear, making it easy to log into our inventory system.
These cells made the rest of the workflow much easier
We purchased this vial to support a neonatal sepsis model project where donor-to-donor variability can derail weeks of work. This batch behaved consistently: minimal spontaneous activation, good recovery, and reliable response curves after stimulation. The biggest win was that the monocytes differentiated into macrophages with uniform adherence and clear phenotype shifts.
Strong experience from ordering through data generation
These cells thawed evenly, maintained good morphology, and reached target density without excessive debris. The positive selection appears well executed-our downstream assays (phagocytosis and antigen uptake) produced tight replicate variance across wells.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.