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Human PB CD14+ Monocytes (Age: 27), 1-5 x 10^7 cells (MTS-1022-JF16)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
1 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

1 x 10^7 cells, 5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What's the recommended approach to reduce variability when working with a smaller lot?

With smaller lots, the main risk is losing cells to over-handling or inefficient plating. We recommend minimizing centrifugation steps, using consistent density, and avoiding frequent medium changes in the first 24 hours unless necessary. Standardize your timing (recovery, differentiation days, stimulation windows) and keep conditions tightly controlled. We can help you design a streamlined workflow that protects cell yield and improves reproducibility.

Do you support add-on macrophage differentiation services for teams with limited bandwidth?

Yes. If your team wants to reduce labor or variability, we can provide differentiated macrophages and optional polarization/validation services with defined deliverables. This is helpful when internal bandwidth is limited or when you need consistent data packages for reporting and decision-making.

What should we expect in terms of turnaround and communication support?

We prioritize clear communication from order through delivery, and we provide practical post-delivery support for thawing and workflow alignment. If you have a fixed project timeline, share it up front-we can coordinate logistics and deliverable expectations so the cells arrive when your lab is ready to execute.

For a small-but-rigorous experiment, this 1.5×10^7 CD14+ monocyte vial delivered exactly what we needed
The thaw yielded good recovery, minimal clumping, and a clean population suitable for both flow cytometry and functional assays. We differentiated the cells into macrophages and observed stable viability through multi-day culture. Our stimulation assays produced clear dynamic ranges and reproducible results across replicates.
We needed an age-27 donor monocyte prep for a comparative analysis and this vial performed well
Differentiation into macrophages was consistent, and marker readouts were stable across wells. The handling guide was especially helpful for optimizing the rest period post-thaw to minimize stress artifacts. This product provided the quality and consistency needed for meaningful comparisons and helped us keep our project timeline on track.
The experience reduced the variability we often see in primary immune cell work
These monocytes were ordered for a co-culture assay with epithelial cells, which can be sensitive to monocyte quality. The cells thawed cleanly and were easy to seed at consistent densities. After differentiation, macrophage behavior was predictable, and we obtained reproducible phagocytosis and cytokine profiles across replicates. The small-format vial made it easy to allocate cells across assay arms without stretching too thin.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.