Online Inquiry

Human PB CD14+ Monocytes (Age: 35), 5 x 10^7 cells (MTS-1022-JF29)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Can these cells be used to generate macrophages for antibody-dependent phagocytosis (ADCP) assays?

Yes. Monocyte-derived macrophages are commonly used for Fc-driven functional assays. The critical success factors are consistent differentiation (timing and cytokines), controlled effector:target ratios, and well-defined target labeling. Include macrophage-only and isotype controls to isolate Fc effects from baseline uptake.

Do you have guidance for maximizing post-thaw recovery?

Rapid thaw, immediate dilution into warm medium, and minimal mechanical stress are key. Avoid harsh pipetting, and use gentle centrifugation if a wash is required. Most importantly, prevent temperature cycling at receipt by transferring to LN2 promptly and planning the thaw workflow before removing vials from cold storage.

How do I confirm the cells are behaving as monocytes before differentiation?

Check morphology (non-adherent/weakly adherent initially), viability, and optionally CD14 expression by flow. If your downstream application is highly sensitive, run a small pilot differentiation to confirm expected marker progression and functional behavior.

Strong performance in Fc-function workflows
We used Age 35 PB CD14+ monocytes to generate macrophages for ADCP-style assays. Differentiation was reliable, and the macrophages showed robust uptake when antibodies were present. The main operational benefit was having enough cells to keep the project within a single donor source, which improved comparability.
Reduced planning friction for large experiments
Having a large cryopreserved vial removed the unpredictability of fresh sourcing. We could plan plate layouts weeks ahead and still maintain consistency. Quality statements and handling notes were aligned with what our team expects.
The product scaled well for multi-condition co-culture plates
Our lab studies macrophage-driven T cell modulation. Starting from these monocytes, we produced macrophages that behaved consistently in co-culture. The key was controlling differentiation timing and media conditions.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.