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Human PB Pan Monocytes (Age: 67), Negative selected, 5 x 10^7 cells (MTS-1022-JF86)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. Monocytes provide an ideal model for the study of macrophage biology and mechanisms.
These pan monocytes are purified from peripheral blood by removing other cell types using immunomagnetic negative selection, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Isolation Method

Negative selection

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What if I need to compare older vs younger donors under identical experimental conditions?

That's a common and powerful design. The highest-impact move is to keep everything else constant: same media, same cytokine lot, identical plate type, and synchronized thaw/differentiation scheduling. If you share the age brackets you want, we can help you match product formats and plan lot strategy so the comparison is meaningful at the macrophage phenotype/function level.

What sterility/contamination controls are performed before release?

Each vial is tested negative for mycoplasma and for bacterial/fungal/yeast contamination. This is particularly important for long macrophage cultures where even low-level contamination can distort cytokine or metabolic readouts.

How should we store and inventory these vials for a multi-month aging project?

Store in liquid nitrogen (-150 °C) and avoid unnecessary tank access events. Many labs assign a dedicated rack/box for a single project and log each vial removal to preserve chain-of-custody. If you want, we can suggest an inventory plan that aligns with experimental milestones.

If you're building age-stratified immune datasets, this product removes a major sourcing bottleneck
The batch fit seamlessly into our standard thawing and differentiation protocols, and the macrophages were functional in phagocytosis assays and cytokine release profiles. The data quality was strong enough to move directly into confirmatory experiments. From an operational perspective, having a defined donor age and negative selection method simplifies internal reporting and method sections for manuscripts.
These monocytes were integrated into a drug mechanism-of-action study evaluating macrophage signaling pathways
We observed consistent pathway activation upon stimulation and reproducible dose-response behavior across replicates. The cells also produced good-quality lysates for downstream western blotting and phosphorylation assays, which can be sensitive to sample variability. The large cell count reduced the need for pooling, which preserved donor-specific biology.
These negative-selected monocytes delivered stable culture behavior and reliable differentiation, with clear treatment-driven changes in marker expression
We ran prolonged culture experiments and did not see sudden viability collapses that can happen with more delicate donor material. The overall handling experience was smooth, and the support team answered our technical questions quickly.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.