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Human PB CD14+ Monocytes (Age: 45), 1 x 10^7 cells (MTS-1022-JF38)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
1 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

1 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What do you recommend for first-day QC after thaw?

A simple first-day QC package is: viability measurement, cell count recovery, a quick morphology check after plating, and (optionally) a minimal flow panel for CD14 at baseline. This helps you confirm the starting condition without consuming too many cells. Once differentiated, you can apply your macrophage marker panel and functional readouts with more confidence.

We're comparing donors across age brackets-how should we control variables when using Age 45 monocytes?

The cleanest strategy is to keep everything except donor constant: thaw workflow, recovery duration, differentiation days, media lot, cytokine concentrations, and stimulation timing. We also suggest running a standardized baseline marker check before differentiation and a benchmark stimulus after differentiation so you can normalize responsiveness across donors. The 1 × 10^7 size is a good "unit" for donor-to-donor comparisons because it naturally encourages single-run, standardized processing.

If we plan to run multiple repeats, should we order multiple 1 × 10^7 vials or move to a larger format?

If your experimental design is iterative (multiple small runs, method optimization, staggered scheduling), multiple 1 × 10^7 vials can be operationally convenient. If you want to execute a large matrix of conditions in one window while minimizing donor variance, moving to 2.5 × 10^7 or 5 × 10^7 formats is often more efficient.

We delivered a client study requiring a small but well-controlled macrophage model
The product documentation around screening and contamination status simplified client reporting and reduced back-and-forth questions. From a delivery standpoint, reliability matters as much as biology, and this order met expectations. We'll continue using this format for focused projects with tight experimental scopes.
A dependable input for controlled innate immune response profiling
This vial was used to generate macrophages for evaluating innate responses to new adjuvant candidates. Recovery and differentiation were consistent, and the macrophages showed clear stimulus-driven responses in our cytokine readouts. The baseline was stable enough that we could detect differences between candidates without excessive normalization.
We often advise teams to start with a smaller vial when standardizing monocyte-to-macrophage differentiation
The culture behaved consistently, and the derived macrophages were suitable for both phenotyping and functional assays. The clear guidance on storage and the emphasis on avoiding repeated freeze-thaw are appreciated-it aligns with best practices and reduces avoidable variability.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.