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Rat Bone Marrow Monocytes (with RFP Expression), 0.5 x 10^6 cells (MTS-1022-JF99)

Datasheet

Overview

Description

With RFP expression, this product is isolated from the bone marrow of pathogen-free laboratory rat.
0.5 x 10^6 cells are delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Rat

Product Type

Immune cells

Specification

Source

Bone marrow

Format

Cryopreserved

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

0.5 x 10^6 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Can I run co-culture experiments and still cleanly identify my monocyte/macrophage population?

Yes. RFP is particularly useful in co-cultures where you need unambiguous separation of macrophages from tumor cells, stromal cells, or immune subsets. We recommend deciding early whether you'll quantify by microscopy, flow cytometry, or both, and then optimizing filters/compensation to keep your RFP gate stable across runs.

How do I avoid spectral overlap and compensation headaches in multi-color panels?

Use single-color compensation controls, choose fluorophores with clear spectral separation, and avoid pairing RFP with dyes that bleed heavily into the same channel. If you share your planned panel, we can propose a practical "low-spillover" setup tailored to your cytometer lasers and detectors.

Can these RFP monocytes be used for macrophage functional assays beyond imaging?

Absolutely. Once differentiated, you can use them in functional readouts (phagocytosis, cytokine response, polarization comparisons) while still retaining a robust tracking handle. For publication-grade data, we recommend reporting both imaging/flow tracking metrics and at least one orthogonal functional endpoint to connect phenotype with function.

This product supports clean, reproducible workflows for tracking primary rat monocytes in complex experimental environments
We ran migration and adhesion studies and found the signal strong enough for real-time imaging with minimal optimization. The cells also maintained typical responsiveness, which matters when you're trying to link behavior to biology rather than labeling artifacts. Packaging and delivery were professional, and the handling instructions were specific.
It's a practical solution for multi-color experimental setups, especially when you want to avoid dye-related variability
We used RFP-expressing rat monocytes to validate a macrophage differentiation workflow alongside GFP-labeled target cells. The two-color design was straightforward, and RFP remained distinct throughout the culture period. The cells differentiated smoothly and retained readable fluorescence, which helped us document morphology changes and evaluate differentiation efficiency. The product reduced our dependence on repeated staining and improved consistency across replicates.
Our group runs tissue-mimetic co-culture systems and frequently needs stable fluorescent markers to track cell fate
The RFP monocytes provided clear visualization within dense matrices where staining can fail or diffuse. The signal-to-background ratio was excellent, and the cells tolerated the matrix conditions better than expected. We could quantify survival and distribution without repeatedly disturbing the culture.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.