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Human PB CD14+ Monocytes (Age: 26), 2.5 x 10^7 cells (MTS-1022-JF14)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
2.5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

2.5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Is 2.5 × 10^7 sufficient for multi-condition polarization studies, and how do you recommend allocating cells?

Yes, 2.5 × 10^7 can support a robust design if allocation is planned carefully. We recommend mapping conditions and timepoints first, then allocating a defined fraction for baseline QC/phenotyping. If you're running both functional and molecular endpoints, consider staging: complete functional assays on a subset and reserve cells for high-value molecular readouts once conditions are confirmed. We can help you model cell usage by plate format and endpoint to maintain statistical power.

Do you provide recommendations for flow cytometry panels for macrophage differentiation verification?

Yes. While panel choices depend on your macrophage state of interest, we can suggest an efficient core panel to confirm monocyte-to-macrophage transition and activation states, plus optional markers aligned to your application. We also advise on timing-some markers are most informative early, others after full differentiation.

Can you support downstream assays like phagocytosis, cytokine release, or chemotaxis?

Absolutely. We can support optional functional characterization and standardized reporting. If your team wants to reduce variability or accelerate decision-making, we can deliver a turnkey package: monocytes,differentiated macrophages, defined stimulation/functional assays with clear deliverables.

We prioritize low-activation monocytes for signaling assays, and these cells delivered
Baseline NF-κB pathway activity was low, giving us crisp induction after stimulation. Differentiation into macrophages was uniform, and the cultures remained healthy across multi-day experiments. The product arrived well packed, and the handling guide aligned with best practices, which helped junior staff execute the protocol confidently.
This vial was purchased as part of an age-matched donor set
Recovery was strong, and the cells remained stable after a short rest period. We were able to differentiate into macrophages and run functional assays with predictable outcomes-phagocytosis, cytokine induction, and marker panels all looked clean. The 2.5×10^7 format helped us avoid overextending cell numbers while still supporting replicates.
We used these monocytes to generate macrophages for a pathogen interaction study
The derived macrophages showed robust uptake and strong stimulus-dependent cytokine responses, while maintaining low background activity-critical for detecting subtle differences between strains. The cultures were uniform, and viability held steady across the experiment.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.