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Human PB CD14+ Monocytes (Age: 40), 5 x 10^7 cells (MTS-1022-JF33)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Can I reserve part of the vial for later without impacting quality?

We do not recommend partial thawing or re-freezing. The best practice is to design your plate map so the full vial is used in one thaw event. If you need multiple runs, consider using multiple vials or selecting smaller vial formats for staged work.

What are the most common reasons macrophage differentiation fails even with good starting monocytes?

Inconsistent seeding density, variable cytokine exposure timing, and media/reagent endotoxin are the top issues. Another frequent culprit is over-handling in the first day after plating. A strict SOP with recorded timestamps solves most failures.

How do you recommend using this vial for a multi-arm macrophage project (polarization + co-culture + secretome)?

Plan a "single differentiation run" and branch into arms at defined time points. Keep one reference condition throughout (standard macrophages) and then apply polarization cues or co-culture partners. For secretome studies, control serum conditions and media change timing strictly-those variables influence background as much as biology.

The scale made that possible without changing donor source, which improved comparability
Differentiation was consistent once we locked down the SOP (timing, density, media changes). Shipping and packaging were reliable, and the LN2 storage guidance was clear and emphasized-helpful for preventing viability loss. This product supported a complex experimental design with fewer surprises.
Delivery and handling guidance were reliable
The cryopreserved format and clear handling notes made it easier to onboard new staff and keep procedures consistent. Scientific performance was solid across repeats. We'll continue using this product for macrophage generation.
Using a large cryopreserved vial helped keep conditions constant and reduced run-to-run drift
Secretome assays can be noisy if baseline activation fluctuates. These monocytes differentiated reliably and produced stable baselines when we controlled media conditions. Results were consistent across replicates, and morphology was stable. The large vial size helped us keep controls strong.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.