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Human PB CD14+ Monocytes (Age: 28), Negative selected, 1 x 10^7 cells (MTS-1022-JF20)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood by removing other cell types using immunomagnetic negative selection, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
1 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Isolation Method

Negative selection

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

1 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

How do you help customers minimize basal activation during shipping and post-thaw handling?

We provide handling guidance that emphasizes gentle recovery, controlled media exposure, and appropriate rest time. For activation-sensitive endpoints, consistency in timing and temperature handling is crucial. We can also suggest simple baseline checks to confirm the cells are stable before stimulation.

Why should I choose negative selection for PB CD14 monocytes instead of positive selection?

Negative selection is often preferred when you want monocytes with minimal direct surface targeting, which can be important for receptor biology, signaling, or sensitive activation studies. This approach aims to enrich monocytes while reducing the risk of altering certain surface epitopes that could confound downstream functional readouts.

Can these cells be used directly in co-culture assays without differentiation?

Yes. Many customers use negatively selected monocytes directly in short-term functional assays, co-culture models, or stimulation studies. We recommend optimizing seeding density and rest period to stabilize the baseline prior to initiating co-culture or stimulation.

We chose the negative selection CD14 monocytes specifically because our assays are extremely sensitive to surface perturbation
The 1 × 10^7 size was convenient for our workflow: we ran phenotype confirmation, a stimulation time-course, and kept an aliquot for RNA. The supplier's guidance was practical (timing, dilution order, gentle mixing), not generic. Overall, the negative selection format fit our method development needs and improved confidence in the data.
This batch performed well-low background activation and consistent responses across replicates once we standardized recovery conditions
We appreciated the supplier's willingness to discuss troubleshooting scenarios like clumping and variable adherence. The documentation was easy to archive and share internally. If your study depends on subtle functional differences rather than huge phenotypic shifts, negative selection is a strong choice and this product delivered reliably.
We purchased the age-28 negative-selected monocytes for a method validation study and were pleased with the overall performance
The cells handled well, and we observed predictable viability and recovery. In our functional assays, the baseline remained controlled and the response to defined stimuli was clear. The documentation was straightforward, and questions were answered quickly with specific, actionable suggestions rather than vague advice.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.