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Human PB CD14+ Monocytes (Age: 33), 5 x 10^7 cells (MTS-1022-JF27)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Are these monocytes compatible with serum-free differentiation?

Many labs successfully differentiate monocytes under defined or low-serum conditions, but outcomes are protocol-dependent. If you go serum-free, validate attachment, survival, and marker expression early in the process. We recommend running a short optimization matrix (cytokine dose × media system) before committing a full vial.

What's the best way to split a vial into multiple experimental arms without bias?

Fully resuspend the cells into a single homogeneous suspension, then aliquot by volume using consistent mixing between dispenses. Avoid "serial plating" without mixing-density gradients can create artificial phenotype differences that masquerade as biological effects.

Our project involves mapping macrophage phenotypes across polarization conditions and time points
Using this vial, we achieved consistent attachment and morphology across plates. We appreciated the QC statements and donor screening list for documentation. The key learning was to minimize handling stress early; once we did that, variability dropped.
Smooth integration into serum-reduced workflows
We run serum-reduced differentiation and worried about attachment and survival. With a small optimization step, the cells performed well and differentiated reliably. The larger cell count gave us room to optimize without sacrificing the full study. Packaging and delivery were robust.
Dependable for co-culture and secretome profiling
We used differentiated macrophages for co-culture with fibroblasts and secretome profiling. The starting monocytes were consistent enough that our secretome differences tracked with experimental variables rather than batch effects. The product documentation supported our internal QA process, and delivery was on schedule.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.