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Human PB CD14+ Monocytes, 1 vial (MTS-1022-JF60)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

1 vial

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

This page says "1 vial" without a cell number in the title-how do I confirm what I'm receiving?

The title indicates "1 vial," and the datasheet/specification section provides the formal product details. If your experiment requires a precise cell input per condition, we recommend aligning your order with your planned seeding calculations and referencing the datasheet for the exact vial content and handling requirements.

Is this product still suitable for macrophage or dendritic-cell generation workflows?

Yes. CD14+ monocytes are commonly used as precursors for macrophage and dendritic-cell differentiation, and a vial-based product is convenient for labs that want a ready-to-thaw starting material for method development, optimization, or smaller-scale differentiation runs.

Can I save time by thawing now and freezing aliquots for later?

We don't recommend that. The product guidance explicitly advises avoiding repeated freeze-thaw cycles. If you need multiple run dates, the best practice is to order multiple vials (or a larger cell-count option) so each experimental run starts from a fresh, properly stored vial.

This vial was used for method development in a new macrophage assay platform
We needed cells that would behave predictably while we optimized seeding density, resting time, and stimulation windows. The CD14+ monocytes performed reliably and tolerated routine handling well. After differentiation, we saw clear polarization responses and low baseline noise in our cytokine readouts.
Our lab runs multiple projects in parallel, so a single-vial option is valuable for rapid feasibility checks
This CD14+ monocyte vial performed as expected: strong recovery, clean differentiation, and robust responses to standard stimuli. The cells attached well and maintained stable culture behavior. We also appreciated professional packaging and clear vial identification, which reduces confusion when multiple experiments are running.
We needed a reliable starting population to produce macrophages quickly, and the cells delivered consistent results without extensive troubleshooting
Differentiation was uniform, and our endpoint flow and cytokine assays were clear and reproducible. Communication on shipping and handling was professional, and the product arrived ready for immediate use.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.