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Human PB CD14+ Monocytes, Positive selected, 1-5 x 10^7 cells (MTS-1022-JF72)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.

These human CD14+ monocytes are purified from peripheral blood using immunomagnetic positive selection directed against CD14, with the highest viability and plating efficiency.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
1 x 10^7 cells per vial.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Isolation Method

Positive selection

Purity

90%, determined by flow cytometry.

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

1 x 10^7 cells, 2 x 10^7 cells, 4 x 10^7 cells, 5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What does "positive selection directed against CD14" mean in practical terms for my assay performance?

It means monocytes are enriched using CD14-targeted immunomagnetic capture, resulting in a CD14-focused population that is typically favored when you need clearer gating and stronger CD14 identity for consistent downstream differentiation or immunophenotyping.

What purity level can I expect, and how is it determined?

This product lists 90% purity, determined by flow cytometry. That gives you a measurable benchmark for planning assay sensitivity and interpreting donor-to-donor variation.

Are these cells suitable for macrophage or dendritic cell differentiation workflows?

Yes. CD14+ monocytes are positioned as readily available precursors for generating macrophages and dendritic cells, and this product is described as offering high viability and plating efficiency, which are exactly the parameters most labs care about at differentiation Day 0.

Everything arrived well protected with documentation that was ready to file
We purchased the positive-selected CD14 monocytes for a set of assays requiring clean monocyte input to minimize cross-talk from other PBMC subsets. The difference was noticeable: less background, clearer separation in functional readouts, and fewer ambiguous intermediate phenotypes after stimulation. The thawing protocol was easy to follow, and recovery was stable.
This has become our preferred format when purity is the priority
The cells were straightforward to handle, and the purity made it easier for new team members to generate clean flow cytometry plots without excessive troubleshooting. Differentiation yielded uniform morphology and consistent marker expression over time. Our functional readouts improved largely because the input population was less heterogeneous.
This positive-selected CD14 monocyte product fit smoothly into our workflow
The cells recovered well post-thaw and showed strong uniformity in our gating strategy, which streamlined data processing across hundreds of wells. Differentiation was efficient and reproducible, and the macrophages responded in a predictable, dose-dependent manner to our stimuli. We also appreciated the reliability of delivery and the professional communication throughout the order. It's a strong option for labs scaling monocyte-based assays.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.