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Human PB CD14+ Monocytes (Age: 42), 2.5 x 10^7 cells (MTS-1022-JF35)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
2.5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

2.5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

For 2.5 × 10^7 cells per vial, what's the best way to plan seeding density across multiple assays?

A practical approach is to back-calculate from your most cell-demanding endpoint (e.g., co-culture, repeated sampling, or multi-panel flow). Many labs allocate separate "buckets" for differentiation, baseline controls, and stimulation conditions to avoid mid-study pooling. With 2.5 × 10^7 cells/vial, you can typically support parallel arms (e.g., M0 differentiation + M1/M2 polarization + QC phenotyping) while still reserving cells for repeat runs.

Can I use these monocytes for dendritic cell generation as well as macrophages?

Yes. CD14+ monocytes are commonly used as precursors for both macrophages and monocyte-derived dendritic cells. The key is to optimize cytokine cocktails, culture duration, and maturation stimuli based on your target phenotype. If you plan to compare macrophage and DC lineages from the same donor source, consistent handling (thaw, recovery, baseline culture) is essential to ensure that observed differences reflect biology rather than processing variation.

What are the most common pitfalls after thaw, and how do you recommend avoiding them?

The top pitfalls are (1) prolonged time at room temperature during thawing/processing, (2) harsh centrifugation or pipetting that increases cell stress, and (3) repeated freeze-thaw or attempted refreezing. We recommend a controlled thaw, gentle handling, and a defined recovery phase before stimulation.

We used these monocytes to generate macrophages for co-culture studies with patient-derived tumor cells
The 2.5 × 10^7 format was convenient: it allowed us to run biological replicates and include sufficient controls without needing multiple vials. Differentiation outcomes were reproducible and the macrophages showed robust responsiveness to polarization stimuli, improving interpretability of our tumor-conditioned media experiments.
This product supported a new assay build
We found the cells easy to handle post-thaw, and they gave clean, interpretable results across multiple readouts. For a small team with limited bandwidth, having a consistent monocyte source is a major efficiency gain. We will likely standardize on this format for future development cycles.
A reliable option for shared research environments
We routinely help multiple groups troubleshoot monocyte-derived macrophage phenotyping, and inconsistent starting material is a common culprit. This monocyte vial performed well and produced clear flow profiles after differentiation and stimulation. The cells tolerated staining and washing steps without excessive loss, and the background signal was manageable.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.