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Mouse Bone Marrow Monocytes (with Luciferase Expression ), 0.5 x 10^6 cells (MTS-1022-JF93)

Datasheet

Overview

Description

With Luciferas expression, this product is isolated from the bone marrow of pathogen-free laboratory C57BL/6 mouse.
0.5 x 10^6 cells are delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Mouse

Product Type

Immune cells

Specification

Source

Bone marrow

Format

Cryopreserved

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

0.5 x 10^6 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

The vial size is 0.5 × 10^6 cells-can I still do multi-condition experiments?

Yes, with smart prioritization. Use miniaturized formats (96/384 well), reduce "nice-to-have" conditions, and reserve a small fraction for a quick standard curve. We can help you map a design that hits your key hypotheses while staying within cell constraints.

How should I design a luciferase assay so the signal is interpretable and comparable between runs?

Build a simple standardization framework: consistent cell number per well, fixed substrate concentration and incubation time, and a calibration curve (serial dilution of cells) on every plate. If you're differentiating into macrophages first, calibrate at the same differentiation day you'll use for the real experiment.

Can you support follow-on functional profiling once I've tracked where the cells go?

Yes. Many teams first use luciferase to quantify kinetics, then switch to phenotype/function assays (cytokines, phagocytosis, antigen presentation, polarization markers) for mechanism. If you want an integrated plan, we can propose a staged workflow so tracking and function tell a consistent story.

The luminescence was reliable and allowed us to optimize assay conditions quickly
The cells remained healthy through differentiation and standard stimulation protocols. The ability to quantify signal in a plate reader substantially improved our workflow efficiency and reduced operator-to-operator variability.
We purchased these cells for a time-course experiment comparing immunomodulatory compounds
The monocytes were robust, recovered consistently, and supported macrophage differentiation with stable performance. The smaller format was cost-effective and ideal for feasibility testing before scaling. This is a smart product when you need sensitive quantification but want to avoid building custom reporter systems in-house.
This product is a highly effective tool
Our team needed a sensitive readout to evaluate macrophage persistence in a complex co-culture environment. These luciferase-expressing monocytes helped us quantify population changes over time without extensive flow cytometry at every time point. The cells differentiated predictably and maintained luminescence during the workflow. Customer support also provided practical recommendations for substrate handling and assay timing.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.