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Human PB CD14+ Monocytes (Age: 55), 5 x 10^7 cells (MTS-1022-JF48)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

How can a 5×10^7 format accelerate a macrophage program with multiple endpoints?

This size is ideal when you need breadth-parallel differentiation conditions, multiple stimuli, time-course sampling, and molecular profiling-while keeping the donor constant. It reduces inter-sample variability and allows you to build a coherent dataset where functional outcomes, phenotyping, and omics readouts all connect back to the same starting population.

Do you provide recommendations for minimizing batch effects when we run large plate sets?

Yes. Batch effects often come from timing drift and inconsistent handling across plates. We recommend a standardized processing schedule, consistent reagent lots, and a plate-randomization strategy for conditions. For omics, aligning extraction timing and using balanced experimental blocks can significantly improve downstream statistics.

What is your guidance on serum choice and its impact on macrophage phenotypes?

Serum source can strongly influence differentiation and activation signatures. We recommend choosing a consistent serum strategy (or serum-free conditions when appropriate), verifying endotoxin levels when cytokine readouts matter, and documenting serum lot information for reproducibility. If you're comparing phenotypes, keep serum constant across all arms.

Large format enabled full-program consistency
That decision reduced variability and strengthened our comparisons across assays. The monocytes recovered well, differentiated consistently, and produced stable macrophage phenotypes over multiple runs. We were able to complete functional tests, imaging, and transcript-level validation without switching materials midstream.
With 5 × 10^7 cells, we could schedule assays weekly without rationing
Recovery and plating were consistent, which improved throughput and reduced plate failures. The derived macrophages were robust during handling-heavy protocols and maintained stable controls, which is essential when running high-throughput stimulus or compound panels. This lot helped our team focus on assay performance and biology rather than primary cell variability.
For labs that value both scientific reliability and operational smoothness, this lot is a solid purchase
Beyond biological performance, the overall experience was professional. Ordering was straightforward, shipping was reliable, and the product arrived in good condition. Handling guidance was clear, and we saw strong recovery when following standard thaw procedures. The macrophages derived from these monocytes were consistent across conditions and timepoints, which is exactly what you want when multiple stakeholders depend on the same dataset.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.