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Human PB CD14+ Monocytes (Age: 59), 5 x 10^7 cells (MTS-1022-JF51)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

How do you recommend designing controls for donor-age-related variability?

For age-focused studies, the best practice is to treat donor age as a biological variable: keep stimulation timing consistent, include baseline (unstimulated) controls, and consider running replicate wells to smooth intrinsic donor-to-donor differences. If your project requires multiple conditions across the same donor, ordering enough vials from the same lot helps maintain continuity across phases.

What shipping/storage conditions are required to maintain performance?

Cells are shipped on dry ice and should be transferred directly to liquid nitrogen storage (-150°C to -190°C) upon receipt. Deviations-especially temporary warming-can reduce viability and distort functional responses. Following storage guidance is essential because viability cannot be guaranteed if handling procedures are not followed.

Can you support downstream characterization beyond the basic product QC?

Absolutely. Many teams request add-ons such as custom flow panels (e.g., CD14 with additional monocyte markers), post-thaw viability checks under their exact medium, or fit-for-purpose functional endpoints. If you share your assay design (stimuli, readouts, time points), we can suggest a practical validation plan to reduce rework.

This is a dependable option with professional support behind it
From an operational standpoint, the experience was smooth: clear ordering, transparent fulfillment, and high-quality packaging. Scientifically, the product performed reliably for monocyte-to-macrophage differentiation and downstream immune phenotyping. The lot behaved consistently across replicates, which reduced our statistical uncertainty and improved confidence in our conclusions.
We ordered the 5×10^7 format to support a multi-plate screen and the yield matched our planning needs
Post-thaw viability supported immediate plating for differentiation, and we were able to run polarization conditions in parallel without scrambling for extra vials. The cells exhibited solid responsiveness to LPS/IFN-γ and IL-4/IL-13 in our endpoint markers and secreted cytokine profiles.
Differentiation into macrophages was uniform, and the cells tolerated media changes without unusual sensitivity
Our lab is picky about cell preparations because we run multiple downstream platforms-flow, qPCR, and imaging-based functional assays. This age-59 CD14+ monocyte lot handled well across the board. It was straightforward to thaw, wash, and seed, and we observed good recovery after an overnight acclimation.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.