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Porcine Bone Marrow Monocytes, 1 x 10^7 cells (MTS-1022-JF95)

Datasheet

Overview

Description

This product is isolated from the bone marrow of pathogen-free laboratory porcine.
1 x 10^7 cells are delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Porcine

Product Type

Immune cells

Specification

Source

Bone marrow

Format

Cryopreserved

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

1 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Can these monocytes be used to generate macrophages for functional assays?

Yes. These porcine monocytes are designed for research-grade downstream culture workflows, including macrophage differentiation and functional readouts (e.g., cytokine response, phagocytosis, polarization studies). Because donor background and lab-specific protocols can affect outcomes, we typically recommend aligning your differentiation cytokines/serum strategy and plating density with your intended assay endpoints to minimize drift and batch effects.

What is the best practice for thawing and preserving viability?

The key is avoiding repeated freeze-thaw cycles and moving the vial directly from dry ice to liquid nitrogen storage upon receipt if you are not using it immediately. When ready to run, thaw quickly, dilute gradually into pre-warmed medium, and remove cryoprotectant promptly according to your SOP. The product page specifically cautions against repeated freezing/thawing and emphasizes correct cold-chain handling for viability.

If I need a larger study, can I maintain consistency across multiple vials or lots?

For scale-up, we recommend planning around experimental segmentation and documenting key variables (thaw recovery, plating density, differentiation window, stimulus dose/time). If you anticipate multi-lot use, we can help you design a bridging plan (e.g., "lot qualification" run with shared controls) to keep your porcine macrophage data comparable over time.

We'll continue using this product for pig macrophage pipeline builds
Thaw-to-plate workflow was smooth, and the cells showed stable behavior across different assay formats (ELISA, microscopy, and basic functional stimulation). The purity and viability were aligned with what we needed for downstream differentiation.
The porcine monocytes performed very well in our polarization experiments
After a brief recovery period, they responded predictably to classic activation cues and generated a clean separation in marker expression by flow cytometry. We also ran RNA extraction for RT-qPCR and obtained high-quality RNA with minimal degradation, which is often a challenge with primary myeloid cells.
This product saved weeks of in-house isolation work
Our lab uses porcine primary cells for macrophage differentiation studies. The monocytes recovered cleanly, with low debris and a very manageable clumping profile compared with other vendors. Differentiation into macrophage-like cells was straightforward and morphology was uniform across the plate, which improved imaging-based quantification.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.