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Human PB CD14+ Monocytes (Age: 32), 5 x 10^7 cells (MTS-1022-JF26)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Can I use these monocytes for co-culture systems (T cells, tumor cells, fibroblasts)?

Yes. CD14+ monocytes are widely used to generate macrophages for co-culture. The key is timing: allow differentiation to stabilize phenotype before introducing partners, and keep media compatibility in mind (serum, cytokines, and antibiotics can all shift macrophage behavior). Include macrophage-only controls on every plate to decouple partner-driven effects from baseline drift.

How do you recommend validating CD14+ identity after thaw?

For critical studies, run a quick flow check on a small aliquot post-thaw (CD14 plus a viability dye). If you're differentiating, consider verifying macrophage markers at day 5-7 (e.g., CD68/CD11b) alongside functional readouts like phagocytosis. This provides a two-step confirmation: starting identity and endpoint phenotype.

Are these suitable for high-throughput screening after differentiation?

They can be, as long as you invest in up-front standardization. High-throughput success depends on consistent differentiation timing, uniform seeding, and plate layout controls (edge effects are real). With a 5 × 10^7 vial, you can create enough macrophages for meaningful HTS-scale pilot panels while keeping donor origin constant within the screen.

It's a strong option when you need volume without switching donors mid-study
We purchased the Age 32 5 × 10^7 PB CD14+ monocytes to support a large macrophage differentiation campaign spanning multiple plates and time points. The cryopreserved format made scheduling predictable. After standardizing our thaw SOP, we achieved consistent attachment and morphology across plates, which improved reproducibility in phagocytosis and cytokine assays.
Helped reduce donor switching headaches
Using a larger vial allowed us to keep the same donor attributes for multiple experimental arms. That improved comparability and reduced re-optimization. The product performed well in polarization and cytokine profiling. We'd buy again for any project where donor consistency is a priority.
This is a dependable product for teams that care about reproducible macrophage starting points
We ran a pilot screen after macrophage differentiation and needed enough cells to populate multiple 96-well plates with controls. This vial size supported that plan without compromising consistency. We appreciated the clear guidance to avoid repeated freeze-thaw and to store properly in LN2. Handling quality directly translated into assay stability.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.