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Human PB Pan Monocytes, Negative selected, 1-5 x 10^7 cells (MTS-1022-JF87)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood by removing other cell types using immunomagnetic negative selection.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
1 x 10^7 cells per vial.

Species

Human

Product Type

Immune cells

Specification

Source

Bone marrow

Isolation Method

Negative selection

Purity

85%, determined by flow cytometry.

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

1 x 10^7 cells, 4 x 10^7 cells, 5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What does "1-5 × 10^7 cells" mean in practice-can I order the exact vial size I need?

Yes. This listing supports multiple vial sizes (e.g., 1×10^7, 4×10^7, 5×10^7), allowing you to match your assay scale without overbuying. Tell us your plate format, replicate count, and whether you're doing direct assays or differentiation, and we'll recommend the most cost-efficient vial configuration.

Can you support differentiation media/attachment optimization so I can get consistent macrophage yield?

Yes. Monocyte-to-macrophage conversion is highly sensitive to early adhesion and cytokine timing. We can recommend attachment-friendly handling steps and media options to improve consistency-especially helpful when you're scaling across plates or running multiple conditions in parallel.

What purity should I expect, and how is it measured?

Purity is provided as a flow cytometry-determined metric (listed as 85% on this page). If your workflow needs higher purity or specific monocyte subsets, we can discuss tighter gating strategies and optional enrichment/characterization steps to align the material with your readout sensitivity.

We ordered this format specifically to test a new set of inhibitors in a macrophage activation model
The monocytes differentiated predictably and showed robust marker shifts after treatment, with minimal variability across wells. RNA yield from treated cells was sufficient for qPCR validation, and we did not encounter unexpected stress-related artifacts. The product arrived on schedule and in good condition.
We had a positive experience with customer support
Our team used this product for generating macrophages used in antibody-mediated functional assays. The cells were stable in culture and the assay performance was consistent across replicates, with clean negative controls. The smaller format reduced waste and allowed us to run a tightly designed experiment without pooling material from multiple sources.
A practical product that delivers clean biology without overcommitting resources
We used it to validate a macrophage differentiation protocol and were pleased with the cell recovery and low baseline activation. Differentiation with M-CSF was consistent, and the resulting macrophages displayed predictable responses to classical and alternative polarization cues. Because the quantity is manageable, it's a cost-effective option for optimizing staining panels, cytokine time courses, or media conditions before scaling up.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.