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Human PB CD14+ Monocytes (Age: 36), 2.5 x 10^7 cells (MTS-1022-JF30)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
2.5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

2.5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

What's your recommendation for viability assessment post-thaw?

Measure viability immediately and again after a short recovery window (e.g., 2-4 hours). Some cells look acceptable immediately after thaw but decline if residual cryoprotectant isn't removed efficiently or if plating stress is high.

Are these suited for antigen presentation studies?

Yes. Monocyte-derived macrophages (and DCs) are common antigen presentation models. For clean results, control differentiation stage, keep stimulation conditions consistent, and include donor-matched unstimulated controls.

How do you minimize variability in downstream polarization markers?

Use fixed seeding density, fixed cytokine timing, and consistent media refresh intervals. Track a small set of "sentinel markers" each run (one surface marker + one secreted cytokine) to detect drift early.

This format is a practical choice
We ran a short optimization of seeding density and cytokine timing and still had enough cells for the full macrophage experiment. Differentiation was reliable and produced consistent phenotype markers. Packaging and delivery were solid, and the storage guidance helped avoid temperature cycling errors.
Reduced waste, improved planning
Large vials can lead to waste for smaller projects. This middle format was efficient-no leftover partial vials, no re-freezing temptation. Performance in differentiation and polarization was strong.
The starting material performed consistently across repeats, and the vial size fit our project scale
We used the differentiated macrophages for cytokine profiling across several stimuli. Results were reproducible and the response dynamic range was good. With careful thawing and consistent plating, we achieved stable baselines.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.