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Human PB CD14+ Monocytes, Negative selected, 1-5 x 10^7 cells (MTS-1022-JF66)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood by removing other cell types using immunomagnetic negative selection.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Isolation Method

Negative selection

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

1 x 10^7 cells, 1.5 x 10^7 cells, 2 x 10^7 cells, 2.5 x 10^7 cells, 4 x 10^7 cells, 5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Why would I choose negative selection for PB CD14+ monocytes instead of positive selection?

Negative selection enriches monocytes by removing other cell types, helping keep your CD14+ cells more "untouched" and reducing the risk of altering surface receptor behavior before you start functional assays. This is especially helpful for studies sensitive to activation artifacts (e.g., baseline cytokines, receptor-driven signaling, polarization sensitivity screens).

How do you confirm that CD14+ enrichment is achieved for this negative-selected product?

The isolated cells are characterized by flow cytometry to ensure CD14+ enrichment, giving you a traceable QC anchor for acceptance checks and internal documentation.

I need a very specific experiment size-what dose flexibility is available here?

This listing provides multiple dose options, including 1×10^7, 1.5×10^7, 2×10^7, 2.5×10^7, 4×10^7, and 5×10^7 cells, enabling you to match study scale without switching product category or isolation approach.

Our study design requires monocytes that haven't been pushed by selection-induced activation
This negative-selected CD14 prep performed well in that context: clean baseline, strong responsiveness when we intentionally stimulated, and consistent differentiation into macrophage-like cells with predictable marker expression.
We ordered this negative-selected CD14 monocyte lot for a macrophage polarization study and found the results highly consistent across donors in our internal reference set
Handling was straightforward, and the cells did not show excessive clumping or debris. Our downstream differentiation and stimulation steps produced clear phenotype separation, which improved our analysis quality and reduced the need for repeated optimization.
In our pipeline, monocytes are used for antigen presentation and innate immune screening
These negative-selected CD14 monocytes arrived with clear QC documentation and performed as expected in culture. Baseline cytokine secretion was low, but the induced response was strong and consistent. We also liked that the product quantity supported multiple assay formats without stretching the cells too thin.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.