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Human PB CD14+ Monocytes (Age: 38), 5 x 10^7 cells (MTS-1022-JF31)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Can these be used to generate macrophages for tumor-associated macrophage (TAM)-like modeling in vitro?

Yes. Monocyte-derived macrophages are a practical foundation for TAM-like polarization (e.g., using tumor-conditioned media or defined cytokines). To keep the model interpretable, include a "reference macrophage" condition (standard M-CSF) and then layer TAM-like cues. This helps distinguish donor baseline from tumor-driven reprogramming.

How do you recommend documenting macrophage generation for reproducibility?

Record thaw timing, recovery steps, seeding density, cytokine concentrations, media change schedule, and endpoint day. Those parameters explain most cross-run variability in macrophage phenotype.

Are these cells suitable for high-content imaging endpoints after differentiation?

Yes. For imaging, consistency comes from uniform seeding and edge-effect controls. Pre-coat plates if your protocol requires enhanced adherence, and maintain consistent media volumes across wells to stabilize morphology and signal intensity.

Stable baseline, strong response range
Our stimulation panels depend on having a stable baseline and a wide dynamic range after stimulation. These monocytes performed well once we standardized thaw and plating. The larger vial size supported replicate-heavy designs.
Operationally dependable for multi-week projects
We ran experiments over several weeks and appreciated being able to keep donor source consistent within the project. Documentation and handling notes were clear and fit our internal QA needs.
Good fit for co-culture buildouts
We generated macrophages for co-culture with tumor cells and fibroblasts. Differentiation was reproducible and downstream functional assays were stable. Overall, a dependable product for complex model systems.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.