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Human PB CD14+ Monocytes (Age: 39), 5 x 10^7 cells (MTS-1022-JF32)

Datasheet

Overview

Description

Monocytes are immune cells that form an important bridge between the innate and adaptive immune response. These cells exist in various phenotypes based on cell surface marker expression and participate in the pathobiology of many systemic diseases. CD14 is known as monocyte differentiation antigen on the surface of myeloid lineage. This protein has a major role in immune recognition and reactivation. CD14+ monocytes are perhaps the most readily available precursors used to generate macrophages and dendritic cells, provide an ideal model for the study of macrophage biology and mechanisms.
These human CD14+ monocytes are purified from peripheral blood, with the highest viability and plating efficiency. Peripheral blood is collected from donors who have screend for Hepatitis B, Hepatitis C, HIV-1, HIV-2, WNV, and CMV.
Isolated cells are characterized flow cytometry to ensure the enrichment of CD14+ cells.
5 x 10^7 cells per vial.
Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Human

Product Type

Immune cells

Specification

Source

(Peripheral blood) PB

Format

Cryopreserved

Disease State

Normal

Donor Attributes

Hepatitis B (-), Hepatitis C (-), HIV-1 (-), HIV-2 (-), WNV (-), CMV (-)

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

5 x 10^7 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

How do you suggest setting acceptance criteria for each thaw?

Define simple metrics: post-thaw viability threshold, recovery yield range, and a morphology/attachment checkpoint after 4-24 hours. For differentiation studies, add a marker checkpoint at a fixed day. This turns each run into a controlled process.

Can these be used for monocyte innate sensing assays directly (pre-differentiation)?

They can, but direct monocyte assays are more sensitive to handling. If your goal is innate sensing (TLR pathways, cytokines), tighten your thaw SOP, minimize processing time, and include early baseline controls to detect handling artifacts.

What's your guidance on avoiding plate edge effects in macrophage assays?

Use consistent volumes, avoid incubator hot spots, and consider reserving outer wells for buffer/media if edge effects are severe. Randomize conditions across the plate rather than grouping all replicates in one area.

Strong starting material for large experimental designs
We needed enough starting cells to support replicate-heavy layouts and multiple time points. This vial size gave us room to do that without switching donors. Differentiated macrophages behaved consistently across plates.
Scientific performance was consistent once handling was standardized
We used a portion for direct monocyte stimulation and the remainder for differentiation. Direct stimulation worked well once we minimized handling time and included early baseline controls.
We run imaging-based macrophage assays and used plate randomization plus consistent volumes
The cells produced stable morphology and signal patterns across plates. This product supported our workflow well.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.