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Mouse Bone Marrow Monocytes (with RFP Expression), 1 x 10^6 cells (MTS-1022-JF94)

Datasheet

Overview

Description

With RFP expression, this product is isolated from the bone marrow of pathogen-free laboratory C57BL/6 mouse .
1 x 10^6 cells are delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma.
Repeated freezing and thawing of cells is not recommended.

Species

Mouse

Product Type

Immune cells

Specification

Source

Bone marrow

Format

Cryopreserved

Quality Control

Cells are negative for mycoplasma, bacteria, fungi and yeast.

Shipping Info

Dry ice

Size

1 x 10^6 cells

Handling Notes

Upon receiving, transfer this product directly from dry ice to liquid nitrogen(-150°C~-190°C) and store it in a liquid nitrogen tank.
We cannot guarantee the cell viability if the cells are not treated appropriately according to handling procedure.

Notes

Avoid repeated freezing & thawing.

Product Disclaimer

Product Disclaimer

This product is provided for research only, not suitable for human or animal use.
We ensure the safety of our products, but we still recommend handling any biological materials with standard precautions as if able to spread infectious disease.

FAQs Customer Reviews Related Products

Can RFP monocytes be used in multiplex imaging with additional fluorophores?

Yes. RFP enables clear two- or three-color strategies when planned thoughtfully. The key is choosing compatible dyes (far-red for nuclei/markers, avoiding overlap with RFP emission), then locking exposure and compensation settings early. We recommend a pilot panel to confirm minimal spillover before scaling up.

Why pick RFP-labeled monocytes instead of GFP-labeled ones?

RFP is a strong choice when your system already contains green signals (GFP reporters in target cells, FITC-based stains, or green biosensors). Moving your monocytes to the red channel simplifies multiplexing and helps you maintain clean spectral separation in imaging and flow.

These monocytes offer a high-confidence, ready-to-use solution
We ordered these RFP+ monocytes for a screening assay where we needed to quantify macrophage presence and behavior across multiple treatment conditions. The fluorescence allowed rapid plate-based imaging and consistent population identification. The cells were robust, differentiated smoothly, and provided reproducible results across repeats. This product saved substantial setup time compared with generating and validating our own reporter lines.
The RFP signal provided a clean handle for sorting and post-sort recovery was strong
After differentiation, the cells maintained healthy morphology and responded predictably to inflammatory stimuli. This helped us build a reliable pipeline for generating fluorescent macrophages without extensive genetic engineering in-house.
A practical, high-utility product for microscopy-driven studies
We used these RFP+ monocytes in a macrophage-tumor cell interaction model where imaging is central. The intrinsic fluorescence was stable and made time-lapse imaging much simpler. The cells tolerated differentiation and maintained RFP expression throughout, enabling consistent tracking in long experiments. This also reduced our dependence on dyes that can leak or alter cell behavior.

For Research Use Only. Do Not Use in Food Manufacturing or Medical Procedures (Diagnostics or Therapeutics). Do Not Use in Humans.